Characterization of bone marrow CD4 to CD8 ratios and lymphocyte composition in adults by image analysis

نویسندگان

چکیده

Bone marrow (BM) lymphocyte subsets are evaluated by flow cytometry or immunohistochemistry for diagnostic purposes; however, CD4:CD8 T ratios often erroneously interpreted using peripheral blood ranges. There few and no recent studies describing the composition of lymphocytes within space, normal reference Lymphocyte in cytopenic patients hospital autopsy BM specimens were to better characterize ratios. Ten with a history cytopenia identified from 2017 2021. Clinical history, cytogenetic testing, results next generation sequencing panel reviewed rule out hematolymphoid disease. Thirty-five decedents who underwent 2018 2019 identified. History disease was ruled chart review. Immunohistochemical staining CD3, CD20, CD4, CD8 digital image analysis. Findings compared group 20 living patients. analysis significantly lower than blood, mean 0.37:1 0.51:1 versus 2.6:1 (p = < .001 both groups). 0.04) found on same specimen, suggesting hemodilution. significant difference when comparing decedents’ marrows > 0.99). Lymphoid aggregates encountered increasing frequency older individuals. These findings aid evaluation distribution evaluation. We also present practical approach

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ژورنال

عنوان ژورنال: Journal of Hematopathology

سال: 2021

ISSN: ['1865-5785', '1868-9256']

DOI: https://doi.org/10.1007/s12308-021-00472-6